Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Amyloid antibody [2E9] (ab252816)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [2E9] to beta Amyloid
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-beta Amyloid antibody [2E9]
    See all beta Amyloid primary antibodies

  • Description

    Rat monoclonal [2E9] to beta Amyloid

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG, HEK-293 and HeLa whole cell lysate. Human brain and hippocampus tissue lysate. IHC-P: Alzheimer’s Disease cerebrum. IP: HeLa whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [2E9] (ab252816)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [2E9] (ab252816)

    Immunohistochemical analysis of paraffin-embedded Alzheimer’s Disease cerebrum tissue labeling beta Amyloid with ab252816
    at 0.583µg/ml, followed by ready to use secondary ab214882. Positive staining on Alzheimer’s Disease cerebrum tissue is observed (PMID: 1640495). The section was incubated with ab252816 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-beta Amyloid antibody [2E9] (ab252816)
    Western blot - Anti-beta Amyloid antibody [2E9] (ab252816)
    All lanes : Anti-beta Amyloid antibody [2E9] (ab252816) at 0.583 µg/ml

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : Human brain tissue lysate
    Lane 5 : Human hippocampus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 87 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 37 secs; Lanes 4-5: 5 secs.

  • Immunoprecipitation - Anti-beta Amyloid antibody [2E9] (ab252816)
    Immunoprecipitation - Anti-beta Amyloid antibody [2E9] (ab252816)

    Beta Amyloid was immunoprecipitated from 1mg of HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate with ab252816 at 19.443. Western blot was performed from the immunoprecipitate using ab252816 at 1/1000 dilution. Goat-anti-Rat IgG for IP (HRP) (ab205720) was used at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10µg (Input).

    Lane 2: ab252816 IP in HeLa whole cell lysate.

    Lane 3: Rat monoclonal IgG instead of ab252816 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 secs.

  • Anti-beta Amyloid antibody [2E9] (ab252816)
    Anti-beta Amyloid antibody [2E9] (ab252816)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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