Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)
![Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)](https://www.abcam.com/ps/products/230/ab230807/Images/ab230807-387017-anti-ippkipk1-antibody-epr2089354-western-blot-wildtype-hek293-ippkipk1-knock-out-hek293-whole-lysates.jpg "Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20893-54] to IPPK/IPK1
- Suitable for: IP, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-IPPK/IPK1 antibody [EPR20893-54]
See all IPPK/IPK1 primary antibodies -
Description
Rabbit monoclonal [EPR20893-54] to IPPK/IPK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HEK-293, IPPK/IPK1 knock out HEK-293, MCF7, Jurkat whole cell lysate. Flow Cyt: MCF7 and HEK-293 cells. IP: MCF7 whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies.
For details on our patents, please refer to www.abcam.com/RabMAbPatents
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20893-54 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)All lanes : Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 (Human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : IPPK/IPK1 knock out HEK-293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were kindly provided by collaborator.
ab230807 was shown to specifically react with IPPK/IPK1 in wild-type HEK-293 cells as signal was lost in IPPK/IPK1 knockout cells. Wild-type and IPPK/IPK1 knockout samples were subjected to SDS-PAGE. ab230807 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200, 000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 37 seconds
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![Flow Cytometry - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)](https://www.abcam.com/ps/products/230/ab230807/Images/ab230807-2-anti-ippkipk1-antibody-epr2089354-flow-cytometry-hek293.jpg)
Flow Cytometry - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (Human embryonic kidney epithelial cell) cells labelling IPPK/IPK1 with ab230807 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)](https://www.abcam.com/ps/products/230/ab230807/Images/ab230807-3-anti-ippkipk1-antibody-epr2089354-immunoprecipitation-mcf7.jpg)
Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)IPPK/IPK1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab230807 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab230807 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab230807 IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230807 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)](https://www.abcam.com/ps/products/230/ab230807/Images/ab230807-387018-anti-ippkipk1-antibody-epr2089354-western-blot-mcf7-jurkat-lysates.jpg)
Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)All lanes : Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds
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![Flow Cytometry - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)](https://www.abcam.com/ps/products/230/ab230807/Images/ab230807-1-anti-ippkipk1-antibody-epr2089354-flow-cytometry-mcf7.jpg)
Flow Cytometry - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling IPPK/IPK1 with ab230807 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)](https://www.abcam.com/ps/products/230/ab230807/Images/ab230807-6-benefits-of-recombinant-antibodies.png)
Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)
