Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)
![Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)](https://www.abcam.com/ps/products/223/ab223532/Images/ab223532-296606-ab183957KOWB.jpg "Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19693] to PI 3 Kinase catalytic subunit alpha/PIK3CA - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free
See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies -
Description
Rabbit monoclonal [EPR19693] to PI 3 Kinase catalytic subunit alpha/PIK3CA - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: F9 and M1 whole cell lysates; Mouse hypothalamus and fetal brain lysates; P0 Rat brain lysate. IP: Mouse hypothalamus lysate.
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General notes
Ab223532 is the carrier-free version of ab183957. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab223532 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19693 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)
This WB data was generated using the same anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody clone [EPR19693] in a different buffer formulation (cat# ab183957).
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PI 3 Kinase catalytic subunit alpha/PIK3CA knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab183957 observed at 125 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab183957 was shown to recognize PI 3 Kinase catalytic subunit alpha/PIK3CA in wild type cells as signal was lost at the expected MW in PI 3 Kinase catalytic subunit alpha/PIK3CA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PI 3 Kinase catalytic subunit alpha/PIK3CA knockout samples were subjected to SDS-PAGE. ab183957 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)](https://www.abcam.com/ps/products/223/ab223532/Images/ab223532-282765-ab183957IP.jpg)
Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)This IP data was generated using the same anti-PI 3 Kinase catalytic sunbunit alpha antibody clone [EPR19693] in a different buffer formulation (cat# ab183957).
PI 3 Kinase catalytic subunit alpha was immunoprecipitated from 0.35 mg of mouse hypothalamus lysate with ab183957 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab183957 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse hypothalamus lysate, 10µg (Input).
Lane 2: ab183957 IP in mouse hypothalamus lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183957 in mouse hypothalamus lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)](https://www.abcam.com/ps/products/223/ab223532/Images/ab223532-3-benefits-of-recombinant-antibodies.png)
Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)
