Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19693] to PI 3 Kinase catalytic subunit alpha/PIK3CA - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free
    See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies

  • Description

    Rabbit monoclonal [EPR19693] to PI 3 Kinase catalytic subunit alpha/PIK3CA - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: F9 and M1 whole cell lysates; Mouse hypothalamus and fetal brain lysates; P0 Rat brain lysate. IP: Mouse hypothalamus lysate.

  • General notes

    Ab223532 is the carrier-free version of ab183957. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223532 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)
    Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)

    This WB data was generated using the same anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody clone [EPR19693] in a different buffer formulation (cat# ab183957).

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: PI 3 Kinase catalytic subunit alpha/PIK3CA knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab183957 observed at 125 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab183957 was shown to recognize PI 3 Kinase catalytic subunit alpha/PIK3CA in wild type cells as signal was lost at the expected MW in PI 3 Kinase catalytic subunit alpha/PIK3CA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PI 3 Kinase catalytic subunit alpha/PIK3CA knockout samples were subjected to SDS-PAGE. ab183957 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)
    Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)

    This IP data was generated using the same anti-PI 3 Kinase catalytic sunbunit alpha antibody clone [EPR19693] in a different buffer formulation (cat# ab183957).

    PI 3 Kinase catalytic subunit alpha was immunoprecipitated from 0.35 mg of mouse hypothalamus lysate with ab183957 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab183957 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse hypothalamus lysate, 10µg (Input).

    Lane 2: ab183957 IP in mouse hypothalamus lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183957 in mouse hypothalamus lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)
    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (ab223532)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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