Human APPL1 (APPL) knockout HeLa cell lysate (ab257836)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262230 - Human APPL1 knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human APPL1 (APPL) knockout HeLa cell lysate (ab257836)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: APPL1 knockout HeLa cell lysate (20µg)
Lane 3: MCF7 cell lysate (20µg)
Lane 4: HEK-293 cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab180140 observed at 90 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab180140 Anti-APPL antibody [EPR13569] was shown to specifically react with Anti-APPL in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab265187 (knockout cell lysate ab257836) lane below 90kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and Anti-APPL knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human APPL1 knockout HeLa cell lysate (ab257836)Homozygous: 2 bp deletion in exon2