Anti-WAPL/FOE antibody (ab70741)
Key features and details
- Rabbit polyclonal to WAPL/FOE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WAPL/FOE antibody
See all WAPL/FOE primary antibodies -
Description
Rabbit polyclonal to WAPL/FOE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human WAPL/FOE aa 1125-1175.
Database link: Q7Z5K2 -
Positive control
- HeLa nuclear extract.
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General notes
This product was previously labelled as WAPL
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WAPL/FOE antibody (ab70741)All lanes : Anti-WAPL/FOE antibody (ab70741) at 0.2 µg/ml
Lane 1 : HeLa nuclear extract at 5 µg
Lane 2 : HeLa nuclear extract at 20 µg
Predicted band size: 133 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
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Western blot - Anti-WAPL/FOE antibody (ab70741)All lanes : Anti-WAPL/FOE antibody (ab70741) at 1/2000 dilution
Lane 1 : Human U2OS cells
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE
Lysates/proteins at 90 µg per lane.
Secondary
All lanes : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
