Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3407(2)] to WAPL/FOE
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-WAPL/FOE antibody [EPR3407(2)]
    See all WAPL/FOE primary antibodies

  • Description

    Rabbit monoclonal [EPR3407(2)] to WAPL/FOE

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293T, and Jurkat cell lysates. HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as WAPL

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
    Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
    All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 133 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
    Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)

    ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.

  • Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
    Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
    All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution

    Lane 1 : Human U2OS cells treated with siRNA control.
    Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
    Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE

    Lysates/proteins at 90 µg per lane.

    Secondary
    Lane 1 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
    Lanes 2-3 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 133 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 120 kDa (possible cross reactivity), 130 kDa (possible cross reactivity), 145 kDa (possible cross reactivity)

    See Abreview

  • Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
    Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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