Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
![Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122482-anti-wapl-antibody-epr34072-western-blot.jpg "Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3407(2)] to WAPL/FOE
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-WAPL/FOE antibody [EPR3407(2)]
See all WAPL/FOE primary antibodies -
Description
Rabbit monoclonal [EPR3407(2)] to WAPL/FOE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, and Jurkat cell lysates. HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as WAPL
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3407(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 133 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122481-anti-wapl-antibody-epr34072-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.
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![Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122480-anti-wapl-antibody-epr34072-western-blot.jpg)
Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution
Lane 1 : Human U2OS cells treated with siRNA control.
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE
Lysates/proteins at 90 µg per lane.
Secondary
Lane 1 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Lanes 2-3 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa (possible cross reactivity), 130 kDa (possible cross reactivity), 145 kDa (possible cross reactivity)
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![Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-4-benefits-of-recombinant-antibodies.png)
Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
