Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15603] to STRAD - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STRAD antibody [EPR15603] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR15603] to STRAD - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Based on the sequence analysis, ab192879 recognizes six isoforms with the predicted MWs of 48KDa, 39KDa, 44KDa, 33KDa, 42KDa and 35KDa, respectively.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    ab232340 is the carrier-free version of ab192879 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232340 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as LYK5

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15603

  • Isotype

    IgG

Images

  • Flow Cytometry - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)
    Flow Cytometry - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling STRAD antibody (red) with purified ab192879 at a dilution of 1/200. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192879).

  • Immunocytochemistry/ Immunofluorescence - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)
    Immunocytochemistry/ Immunofluorescence - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized A549 cells labeling STRAD with ab192879 at a 1/400 dilution, followed by Goat anti rabbit IgG(Alexa Fluor® 488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain: Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192879).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling STRAD with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192879).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STRAD with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192879).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)
    Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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