Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP263Y] to IRS1
Suitable for: Flow Cyt, WB, ICC/IF, IHC-P
Reacts with: Human

Product name

Anti-IRS1 antibody [EP263Y]
See all IRS1 primary antibodies
Description

Rabbit monoclonal [EP263Y] to IRS1
Host species

Rabbit
Specificity

The immunogen used for this product shares 78% homology with IRS2. Cross-reactivity with this protein has not been confirmed experimentally.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human IRS1 (C terminal). The exact sequence is proprietary.
Positive control
- WB: HeLa and HEK293 cell lysates. IHC-P: Human breast carcinoma and cervical carcinoma tissues. ICC/IF: MCF-7 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP263Y
Isotype

IgG
Research areas

Western blot - Anti-IRS1 antibody [EP263Y] (ab40777)

Anti-IRS1 antibody [EP263Y] (ab40777) at 1/10000 dilution (purified) + HEK293 whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 170 kDa
Observed band size: 170 kDa

Blocking and dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] (ab40777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling IRS1 with purified ab40777 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody [EP263Y] (ab40777)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling IRS1 with purified ab40777 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Western blot - Anti-IRS1 antibody [EP263Y] (ab40777)

Anti-IRS1 antibody [EP263Y] (ab40777) (purified) + HeLa whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 170 kDa
Observed band size: 170 kDa

Blocking and dilution buffer: 5% NFDM/TBST
Flow Cytometry - Anti-IRS1 antibody [EP263Y] (ab40777)

Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling IRS1 with unpurified ab40777 at 1/90 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-IRS1 antibody [EP263Y] (ab40777)

Anti-IRS1 antibody [EP263Y] (ab40777) at 1/1000 dilution (unpurified) + HeLa cell lysate at 10 µg

Predicted band size: 170 kDa
Observed band size: 170 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] (ab40777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labelling IRS1 with unpurified ab40777.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-IRS1 antibody [EP263Y] (ab40777)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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