Anti-IRS1 (phospho Y896) antibody (ab4873)
Key features and details
- Rabbit polyclonal to IRS1 (phospho Y896)
- Suitable for: WB
- Reacts with: Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-IRS1 (phospho Y896) antibody
See all IRS1 primary antibodies -
Description
Rabbit polyclonal to IRS1 (phospho Y896) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Chinese hamster
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Immunogen
Synthetic peptide derived from a region of IRS 1 that contains tyrosine 896. The sequence is conserved in human, mouse, and rat.
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Positive control
- Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1 and stimulated with insulin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate 1 (IRS 1). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRS1 (phospho Y896) antibody (ab4873)
Peptide Competition: Extracts prepared from CHO-T cells transiently transfected with human IRS-1 and treated with 50 nM insulin for 5 minutes were resolved by SDSPAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50
µ g/mL ab4873 antibody, following prior incubation with: no peptide (1), a generic phosphotyrosine containing peptide (2), the non-phosphopeptide corresponding to the immunogen (3), or the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4873 blocks the antibody signal, thereby demonstrating the specificity of the antibody.Phosphatase Stripping: Extracts prepared from CHO-T cells transiently transfected with human IRS 1 and
