Anti-KLLN antibody [EPR16934] (ab197892)
![Anti-KLLN antibody [EPR16934] (ab197892)](https://www.abcam.com/ps/products/197/ab197892/Images/ab197892-240324-anti-klln-antibody-epr16934-western-blot.jpg "Anti-KLLN antibody [EPR16934] (ab197892)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16934] to KLLN
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-KLLN antibody [EPR16934]
See all KLLN primary antibodies -
Description
Rabbit monoclonal [EPR16934] to KLLN -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, MCF7, HCT116, Raw264.7, NIH3T3, PC12, C6 whole cell lysates. Human fetal brain and kidney tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16934 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KLLN antibody [EPR16934] (ab197892)All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Exposure time: 3 minutes
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![Western blot - Anti-KLLN antibody [EPR16934] (ab197892)](https://www.abcam.com/ps/products/197/ab197892/Images/ab197892-240311-anti-klln-antibody-epr16934-western-blot.jpg)
Western blot - Anti-KLLN antibody [EPR16934] (ab197892)All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Exposure time: 3 minutes
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![Western blot - Anti-KLLN antibody [EPR16934] (ab197892)](https://www.abcam.com/ps/products/197/ab197892/Images/ab197892-240309-anti-klln-antibody-epr16934-western-blot.jpg)
Western blot - Anti-KLLN antibody [EPR16934] (ab197892)All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 20 kDa
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST
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![Anti-KLLN antibody [EPR16934] (ab197892)](https://www.abcam.com/ps/products/197/ab197892/Images/ab197892-4-benefits-of-recombinant-antibodies.png)
Anti-KLLN antibody [EPR16934] (ab197892)
