Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-APC antibody [ALi 12-28] (ab58)

Key features and details

  • Mouse monoclonal [ALi 12-28] to APC
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-APC antibody [ALi 12-28]
    See all APC primary antibodies

  • Description

    Mouse monoclonal [ALi 12-28] to APC

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse

  • Immunogen

    Amino acids 1-433 N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) fused to maltose binding protein.

  • Epitope

    ALI-12-28 was epitope mapped by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test and was found to bind to APC in the region between nucleotides 135 and 422 (exons 2-3) (Efstathiou et al.).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-APC antibody [ALi 12-28] (ab58)
    Flow Cytometry - Anti-APC antibody [ALi 12-28] (ab58)

    Overlay histogram showing HCT116 cells stained with ab58 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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