Anti-APC antibody (ab265597)
Key features and details
- Rabbit polyclonal to APC
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APC antibody
See all APC primary antibodies -
Description
Rabbit polyclonal to APC -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human
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Immunogen
Synthetic peptide within Human APC aa 2793-2843. The exact sequence is proprietary. NP_000029.2
Database link: P25054 -
Positive control
- IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue.
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General notes
Anti-APC antibody (ab265597) is not recommended for WB of crude lysate. Use at 1 μg/ml to blot enriched sources (e.g. Immunoprecipitates) of APC.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody (ab265597)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling APC with ab265597 at 1/1000 dilution. Detection: DAB.
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Immunoprecipitation - Anti-APC antibody (ab265597)APC was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab265597 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitates using ab265597 at 1 µg/ml .
Lane 1: ab265597 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
