Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP701Y] to APC - BSA and Azide free
  • Suitable for: IP, ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-APC antibody [EP701Y] - BSA and Azide free
    See all APC primary antibodies

  • Description

    Rabbit monoclonal [EP701Y] to APC - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is predicted to detect isoform 2 (short) of APC based on sequence analysis.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Mouse
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, C2C12, and C6 whole cell lysates; ICC/IF: HEK-293 cells; Flow Cyt: HEK-293 cells; IP: HEK-293 whole cell lysate; IHC-P: Human, mouse and rat colon tissues; human colon carcinoma tissue.

  • General notes

    ab239828 is the carrier-free version of ab40778 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239828 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Western blot - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    All lanes : Anti-APC antibody [EP701Y] (ab40778) at 1/5000 dilution (Purified)

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 312 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed represents the truncated APC as described in PMID: 17595655.

    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Immunocytochemistry - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Immunocytochemistry - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

    Immunocytochemistry/ Immunofluorescence analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling APC with Purified ab40778 at 1:100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Immunoprecipitation - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Immunoprecipitation - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

    Purified ab40778 at 1:70 dilution (2μg) immunoprecipitating APC in HEK-293 whole cell lysate.
    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab40778 + HEK-293 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40778 in HEK-293 whole cell lysate.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 160 kDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Flow Cytometry - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Flow Cytometry - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

    Purified ab40778 staining APC in the human cell line 293 (human embryonic kidney) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling APC with purified ab40778 at 1/1000 dilution (1.383 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Immunoprecipitation - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Immunoprecipitation - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

    Unpurified ab40778 at 1/70 dilution (2µg) immunoprecipitating APC in HEK-293 whole cell lysate.
    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab40778 + HEK-293 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40778 in HEK-293 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 160 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling APC with purified ab40778 at 1/1000 dilution (1.383 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling APC with purified ab40778 at 1/1000 dilution (1.383 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

    ab40778 (1:50), unpurified, staining human colon carcinoma by immunohistochemistry, paraffin-embedded tissue. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40778).

  • Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)
    Anti-APC antibody [EP701Y] - BSA and Azide free (ab239828)

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