anti-Infliximab ELISA Kit (Remicade®) (ab237656)
Key features and details
- Sensitivity: 15 ng/ml
- Range: 62 ng/ml - 500 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
-
Product name
anti-Infliximab ELISA Kit (Remicade®)
See all Infliximab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
15 ng/ml -
Range
62 ng/ml - 500 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
anti-Infliximab ELISA Kit (Remicade®) (ab237656) is designed to quantify/measure the antibody against Infliximab with high specificity and sensitivity in biological matrices.
Infliximab (Remicade®) is a therapeutic chimeric monoclonal antibody against tumor necrosis factor (TNF) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Infliximab drug inhibits the action of TNF and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug. Infliximab is used for the treatment of psoriasis, Crohn’s disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis, ulcerative colitis. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Assay Buffer 1 x 50ml Confirmation Reagent 1 x 12ml Infliximab Standard S1 1 x 1ml Infliximab Standard S2 1 x 1ml Infliximab Standard S3 1 x 1ml Infliximab Standard S4 1 x 1ml Infliximab Standard S5 1 x 1ml Infliximab Standard S6 1 x 1ml Infliximab Standard S7 1 x 1ml Micro ELISA Plate 1 unit Peroxidase Conjugate 1 x 12ml Plate Sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Relevance
Infliximab (Remicade®) is a chimeric monoclonal antibody and used to treat auto- immune disorders. Infliximab reduces the amount of active human tumour necrosis factor alpha (hTNFa) in the body by binding to it and preventing it from signaling the receptors for TNFa on the surface of various cell types. TNFa is one of the key cytokines that triggers and sustains the inflammatory reactions. Infliximab is used for the treatment of psoriasis, Crohn’s disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis, ulcerative colitis. -
Alternative names
- human tumour necrosis factor alpha
- Remicade
Images
-
Standard Curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.
