Recombinant human MDM2 protein (Active) (ab271608)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human MDM2 protein (Active)
See all MDM2 proteins and peptides -
Biological activity
50 µl of reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and Mdm2 (0-0.5µg) in 50 mM Tris pH 7.4) was incubated for 30 min. at 37°C. The reaction mixture was transferred to a streptavidin-coated plate and incubated for additional 30 min at 37°C. Anti-Mdm2 and HRP-labeled secondary antibody were incubated with the reaction mixture for 1 hour each, followed by chemiluminescent detection.
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Purity
>= 70 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
82 kDa -
Amino acids
2 to 497 -
Tags
GST tag N-Terminus
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Preparation and Storage
- ACTFS
- Double minute 2 protein
- E3 ubiquitin-protein ligase Mdm2
Images
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Functional Studies - Recombinant human MDM2 protein (Active) (ab271608)Specific activity of ab271608.
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SDS-PAGE - Recombinant human MDM2 protein (Active) (ab271608)SDS-PAGE analysis of 1.5 µg ab271608.