ChIP Kit - One Step (ab117138)
Key features and details
- Assay type: Quantitative
- Assay time: 5 hr
Overview
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Product name
ChIP Kit - One Step
See all ChIP Kit kits -
Assay type
Quantitative -
Assay time
5h 00m -
Product overview
Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.
Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.
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Notes
Primers
The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.
If additional control primers are needed, they are available for purchase as ab171517 (reverse primer) and ab171518 (forward primer).
ChIP assay products and guides
Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.
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Tested applications
Suitable for: ChIPmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Assay Strips (with Frame) 6 units 12 units 8-Well Strip Caps 6 units 12 units 96-Well PCR Plate 1 unit 1 unit Adhesive Covering Film 1 unit 2 units Anti-RNA Polymerase II (1 mg/ml) 1 x 8µl 1 x 16µl ChIP Buffer 1 x 6ml 1 x 12ml DNA Release Buffer ECP3 1 x 14ml 1 x 28ml GAPDH Primer - Forward (20 µM) 1 x 8µl 1 x 16µl GAPDH Primer - Reverse (20 µM) 1 x 8µl 1 x 16µl Non-Immune IgG (1 mg/ml) 1 x 10µl 1 x 20µl Proteinase K (10 mg/mL) 1 x 55µl 1 x 110µl Sonication Frame 1 unit 1 unit -
Research areas
Images
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ChIP - ChIP Kit - One Step (ab117138)
Chromatin was prepared from HeLa cells following Abcam's X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. ChIP was performed with ab8580 (Histone H3 tri-methyl K4) using ab117138. No antibody was added to the blank (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primes and probes are located in the first Kb of the transcribed region.
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ChIP - ChIP Kit - One Step (ab117138)The analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit - One Step, with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.
