ChIP Kit - Plants (ab117137)
Key features and details
- Assay type: Quantitative
- Assay time: 6 hr
Overview
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Product name
ChIP Kit - Plants
See all ChIP Kit kits -
Assay type
Quantitative -
Assay time
6h 00m -
Species reactivity
Reacts with: Plants -
Product overview
Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.
Abcam's ChIP Kit - Plants (ab117137) offers an advantageous tool for identifying direct genome-wide associations between specific regulatory proteins and their target genes in plant cells within 6 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing and southern blot, as well as DNA microarray.
This kit is designed for 24 or 48 ChIP reactions, not for 24 or 48 samples. The standard protocol of the kit allows for performing 8 reactions with one sample. For testing more samples, the amount of each sample should be reduced. The amount of each reagent used for chromatin preparation should be also proportionally reduced.
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Notes
ChIP assay products and guides
Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.
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Tested applications
Suitable for: ChIPmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 24 tests 48 tests 100X Protease Inhibitor Cocktail 1 x 25µl 1 x 50µl 5X Lysis Buffer I 1 x 12ml 1 x 24ml 8-Well Assay Strips (with Frame) 3 units 6 units 8-Well Strip Caps 3 units 6 units Antibody Buffer 1 x 15ml 1 x 30ml Anti-H3K9me2 (1 mg/mL) 1 x 5µl 1 x 8µl Binding Buffer 1 x 5ml 1 x 8ml ChIP Dilution Buffer 1 x 2ml 1 x 6ml DNA Release Buffer 1 x 2ml 2 x 2ml Elution Buffer 1 x 0.6ml 1 x 1.2ml F-Collection Tube 30 units 50 units F-Spin Column 30 units 50 units Lysis Buffer II 1 x 3ml 1 x 6ml Lysis Buffer III 1 x 2ml 1 x 4ml Lysis Buffer IV 1 x 1.5ml 1 x 5ml Normal Mouse IgG (1 mg/mL) 1 x 10µl 1 x 10µl Proteinase K (10 mg/mL) 1 x 25µl 1 x 50µl Reverse Buffer 1 x 2ml 2 x 2ml Wash Buffer 1 x 28ml 2 x 28ml -
Research areas
