Anti-CaMKI (phospho T177) antibody (ab62215)
Key features and details
- Rabbit polyclonal to CaMKI (phospho T177)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CaMKI (phospho T177) antibody
See all CaMKI primary antibodies -
Description
Rabbit polyclonal to CaMKI (phospho T177) -
Host species
Rabbit -
Specificity
ab62215 detects CaMKI only when phosphorylated at threonine 177. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Synthetic phosphopeptide derived from human CaMKI around the phosphorylation site of threonine 177 (L-S-TP-A-C).
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Positive control
- HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab62215 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CaMKI (phospho T177) antibody (ab62215)Immunofluorescence analysis of HeLa cells, using ab62215 (1/500 - 1/1000) staining CaMKI; in the presence and absence of immunizing peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI (phospho T177) antibody (ab62215)Ab62215 staining human normal occipital cortex. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and ampli
