Mouse MIF ELISA Kit (ab209885)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MIF ELISA Kit
See all MIF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse serum 5 13.6% Inter-assay Sample n Mean SD CV% Mouse serum 3 9.8% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
28 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 110 106% - 117% Cell culture extracts 111 108% - 113% Tissue Extracts 108 102% - 118% Hep Plasma 112 105% - 117% EDTA Plasma 109 105% - 112% Cit plasma 112 112% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MIF ELISA Kit (ab209885) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIF protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, tissue extracts, and tissue homogenate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MIF with 28 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Macrophage migration inhibitory factor (MIF) is a 12.37 kDa pro-inflammatory cytokine and is involved in the innate immune response to bacterial pathogens. MIF assembles into a trimer, composed of three identical subunits. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. MIF counteracts the anti-inflammatory activity of glucocorticoids and exhibits phenylpyruvate tautomerase and dopachrome tautomerase activity. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse MIF Capture Antibody 1 x 600µl 10X Mouse MIF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse MIF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
Involvement in disease
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
Sequence similarities
Belongs to the MIF family. -
Cellular localization
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
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Alternative names
- GIF
- GLIF
- Glycosylation inhibiting factor
see all -
Database links
- Entrez Gene: 17319 Mouse
- SwissProt: P34884 Mouse
- Unigene: 2326 Mouse
Images
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Other - Mouse MIF ELISA Kit (ab209885)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse MIF standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse MIF standard curve in 1X Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MIF in mouse serum, plasma and cell culture supernatant samplesThe concentrations of MIF were measured in duplicates, interpolated from the MIF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.5%, plasma (citrate) 2%, plasma (EDTA) 2%, plasma (heparin) 2%, and stimulated RAW 264.7 cell culture supernatant 0.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIF concentration was determined to be 1.06 µg/mL in serum, 286 ng/mL in plasma (citrate), 229 ng/mL in plasma (EDTA), 293 ng/mL in plasma (heparin), and 1.75 µg/mL in LPS-stimulated RAW 264.7 cell culture supernatant.
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Interpolated concentrations of native MIF in RAW 264.7 cell extract and mouse spleen based on 0.49 µg/mL and 3.91 µg /mL extract loads, respectivelyThe concentrations of MIF were measured in duplicate and interpolated from the MIF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIF concentration was determined to be 9.8 µg/mL in RAW 264.7 cell extract and 1.1 µg/mL in mouse spleen extract.
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Sandwich ELISA - Mouse MIF ELISA Kit (ab209885)To learn more about the advantages of recombinant antibodies see here.
