Recombinant mouse IL-10 protein (Active) (ab256035)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at room temperature. Store at -20°C.
Constituent: 0.16% Sodium phosphate
0.2 micron filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in sterile water to 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Images
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Functional Studies - Recombinant IL-10 protein (ab256035)
MC/9 cell proliferation. ≤5 ng/mL; ≥ 2.0 x 10E5 units/mg.
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SDS-PAGE - Recombinant IL-10 protein (ab256035)SDS-PAGE analysis of ab256035 (1μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions. ab256035 has a predicted weight of 18.9kDa.
4-20% Tris-Glycine gel. Coomassie Blue staining.
