Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IL-10 antibody [EPR1114] (ab133575)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1114] to IL-10
Suitable for: Flow Cyt, ICC/IF, WB
Reacts with: Human, Recombinant fragment

Product name

Anti-IL-10 antibody [EPR1114]
See all IL-10 primary antibodies
Description

Rabbit monoclonal [EPR1114] to IL-10
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide within Human IL-10 aa 1-100. The exact sequence is proprietary.
Database link: P22301
(Peptide available as ab169704)
Positive control
- Flow Cyt: HeLa cells; Human PBMCs. WB: recombinant IL-10 protein, Ramos, THP1 and THP1 treated with LPS cell lysates ICC/IF: Human peripheral blood mononuclear cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 3.70 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1114
Isotype

IgG
Research areas

Flow Cytometry - Anti-IL-10 antibody [EPR1114] (ab133575)

Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab133575 at a dilution of 1 in 300 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575) This image is courtesy of an anonymous Abreview

Unpurified ab133575 staining IL-10 in human peripheral blood mononuclear cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin in PBS and blocked with 4% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
See Abreview
Western blot - Anti-IL-10 antibody [EPR1114] (ab133575)

Lane 1 : Anti-IL-10 antibody [EPR1114] (ab133575) (purified)
Lanes 2-3 : purified

Lane 1 : THP-1 treated with LPS cell lysate
Lane 2 : untreated THP-1 cell lysate
Lane 3 : Ramos cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 20 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)

Immunofluorescence staining of Molt-4 cells with purified ab133575 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab133575 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Western blot - Anti-IL-10 antibody [EPR1114] (ab133575)

All lanes : Anti-IL-10 antibody [EPR1114] (ab133575) at 1/1000 dilution (unpurified)

Lane 1 : LPS-treated THP1 cell lysate
Lane 2 : THP1 cell lysate
Lane 3 : Ramos cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Western blot - Anti-IL-10 antibody [EPR1114] (ab133575)

Anti-IL-10 antibody [EPR1114] (ab133575) at 1/1000 dilution (unpurified) + Recombinant IL-10 protein at 0.005 µg

Secondary
Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Flow Cytometry - Anti-IL-10 antibody [EPR1114] (ab133575)

Flow cytometry analysis of permeabilized HeLa cells labelling IL-10 using unpurified ab133575 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).
OI-RD Scanning - Anti-IL-10 antibody [EPR1114] (ab133575)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Flow Cytometry - Anti-IL-10 antibody [EPR1114] (ab133575) This image is courtesy of an anonymous Abreview.

Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) treated with PHA labeling IL-10 with ab133575 at 1/100 dilution (green), followed by Goat anti-rabbit Alexa Fluor^® 488.

1 (dashed line) = isotype control, 2 (black line) = PBMCs untreated, 3 (green line) = PBMCs treated with PHA.
See Abreview
Anti-IL-10 antibody [EPR1114] (ab133575)

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