Anti-IL-10 antibody (ab187251)
Key features and details
- Rabbit polyclonal to IL-10
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-10 antibody
See all IL-10 primary antibodies -
Description
Rabbit polyclonal to IL-10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human IL-10 recombinant protein; U937 treated with LPS lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL-10 antibody (ab187251)Anti-IL-10 antibody (ab187251) at 1/1000 dilution + Human IL-10 recombinant protein at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa (possible dimer)Blocking/Dilution Buffer: 5% NFDM/TBST.
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Western blot - Anti-IL-10 antibody (ab187251)All lanes : Anti-IL-10 antibody (ab187251) at 1/1000 dilution
Lane 1 : U937 (Human histiocytic lymphoma cells) treated with LPS lysate
Lane 2 : Untreated U937 (Human histiocytic lymphoma cells) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking/Dilution Buffer: 5% NFDM/TBST.
The decreased mol mass observed is due to the removal of the signal peptide.
