Factor XIIIa Assay Kit (ab241013)
Key features and details
- Detection method: Colorimetric
- Sample type: Plasma, Purified protein
Overview
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Product name
Factor XIIIa Assay Kit
See all Factor XIIIa kits -
Detection method
Colorimetric -
Sample type
Plasma, Purified protein -
Product overview
The Factor XIIIa Assay Kit (ab241013) utilizes the transglutaminase activity of factor XIIIa to cross link an amine-containing substrate to glutamine-containing substrate resulting in the loss of ammonia which can be quantitatively measured by a colorimetric assay. The kit is easy-to-use and can detect Factor XIIIa (as low as 0.1 Loewy U) from plasma and purified protein samples.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests FXIIIa Activation Buffer (4X) 1 x 2.5ml FXIIIa Assay Buffer 1 x 2.5ml FXIIIa Detection Buffer (2X) 1 x 5ml FXIIIa Inhibitor (Iodoacetamide) 1 vial FXIIIa Probe 1 x 0.2ml FXIIIa Reaction Buffer (4X) 1 x 2.5ml Human Factor XIIIa (0.5 Loewy U/µl) 1 x 20µl NH4Cl Standard (10 mM) 1 x 100µl -
Research areas
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Function
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. -
Involvement in disease
Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. -
Post-translational
modificationsThe activation peptide is released by thrombin. -
Cellular localization
Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma. - Information by UniProt
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Alternative names
- bA525O21.1 (coagulation factor XIII, A1 polypeptide)
- Coagulation factor XIII A chain
- Coagulation factor XIII A1 polypeptide
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Images
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Kinetic progressive curve.Kinetic progressive curves for different amounts of FXIIIa Enzyme.
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Kinetic progressive curve.Kinetic progressive curves for Plasma Samples with and without Iodoacetamide control. -
Standard curves for NH4Cl.Standard curves for NH4Cl (n = 3). -
Estimation of FXIIIa activity in plasma samples.Standard curves for NH4Cl was used to estimate FXIIIa activity in plasma samples (n = 3).
