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Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

Key features and details

  • Mouse monoclonal [131C3] to Lamin A + Lamin C - Nuclear Envelope Marker
  • Suitable for: Flow Cyt, IHC-Fr
  • Reacts with: Human, Pig
  • Isotype: IgG1

Overview

  • Product name

    Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker
    See all Lamin A + Lamin C primary antibodies

  • Description

    Mouse monoclonal [131C3] to Lamin A + Lamin C - Nuclear Envelope Marker

  • Host species

    Mouse

  • Specificity

    ab8984 reacts with lamins A and C. Lamins do not appear to be universally distributed among different cell and tissue types. ab8984 has been shown to react with HT1080 cells in Western blot. Other cell/tissue types have not been tested. Customer feedback seems to indicate that ab8984 does not detect Lamin A+C in rat brain lysates (please see AbReviews).

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Human
    Pig
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Rat Lamin A + Lamin C aa 319-566. Immunogen is purified rat liver lamins.
    Database link: P02545

  • Epitope

    Between residues 319-566.

  • Positive control

    • IHC-Fr: Human colon tissue; Pig skin tissue Flow Cyt: HeLa cells.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)
    Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

    IHC-Fr of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)
    Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

    ab8984 (1/500) on frozen sections of pig skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.

  • Flow Cytometry - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)
    Flow Cytometry - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

    Overlay histogram showing HeLa cells stained with ab8984 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481)  / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8984, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)
    Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

    ab8984 on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.

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