FITC Anti-Lamin A + Lamin C antibody [WL4G10] (ab252002)
![FITC Anti-Lamin A + Lamin C antibody [WL4G10] (ab252002)](https://www.abcam.com/ps/products/252/ab252002/Images/ab252002-1-anti-lamin-a-lamin-c-immmunocytochemistry-wild-type-hap1-cells-human.jpg "FITC Anti-Lamin A + Lamin C antibody [WL4G10] (ab252002)")
Key features and details
- FITC Mouse monoclonal [WL4G10] to Lamin A + Lamin C
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-Lamin A + Lamin C antibody [WL4G10]
See all Lamin A + Lamin C primary antibodies -
Description
FITC Mouse monoclonal [WL4G10] to Lamin A + Lamin C -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Recombinant fragment (GST-tag) corresponding to Human Lamin A + Lamin C (C terminal). (C-terminal fragment of Lamin A)
Database link: P02545 -
Positive control
- ICC/IF: Wild-type HAP1 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
WL4G10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-Lamin A + Lamin C antibody [WL4G10] (ab252002)
ab252002 staining Lamin A + C in wild-type HAP1 cells (top panel) and LMNA knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab252002 at 1/100 dilution (shown in green) and ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in wild-type HAP1 cells fixed with 100% methanol (5 min).
