Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3864(2)] to ERG - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB, IP
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-ERG antibody [EPR3864(2)] - BSA and Azide free
    See all ERG primary antibodies

  • Description

    Rabbit monoclonal [EPR3864(2)] to ERG - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab174739 is the carrier-free version of ab133264. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab174739 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)

    Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133264).

  • Flow Cytometry - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    Flow Cytometry - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)

    ab133264 staining ERG in the human cell line MCF-7(human breast carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/210. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)

    Immunohistochemical staining of paraffin embedded human colonic carcinoma with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133264).

  • Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)

    Immunofluorescence staining of MCF7 cells with purified ab133264 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab133264 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133264).

  • Immunoprecipitation - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    Immunoprecipitation - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)

    ab133264 (purified) at 1/30 immunoprecipitating ERG in HEK293 cells (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)

    Immunohistochemical analysis of paraffin embedded human prostate adenocarcinoma tissue labelling ERG with ab133264 at  a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133264).

  • OI-RD Scanning - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    OI-RD Scanning - Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133264).

  • Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)
    Anti-ERG antibody [EPR3864(2)] - BSA and Azide free (ab174739)

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