Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERG antibody [EPR3864(2)] (ab133264)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3864(2)] to ERG
  • Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-ERG antibody [EPR3864(2)]
    See all ERG primary antibodies

  • Description

    Rabbit monoclonal [EPR3864(2)] to ERG

  • Host species

    Rabbit

  • Specificity

    This antibody does not cross-react with Fli1.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ERG aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11308

  • Positive control

    • Jurkat whole cell lysate (ab7899), 293T cell lysate, MCF7 cell lysate, ERG recombinant protein, Human prostate adenocarcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Flow Cytometry - Anti-ERG antibody [EPR3864(2)] (ab133264)

    ab133264 staining ERG in the human cell line MCF-7(human breast carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/210. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Western blot - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Western blot - Anti-ERG antibody [EPR3864(2)] (ab133264)
    All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/2000 dilution (purified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HEK293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] (ab133264)

    Immunohistochemical staining of paraffin embedded human colonic carcinoma with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] (ab133264)

    Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864(2)] (ab133264)

    Immunofluorescence staining of MCF7 cells with purified ab133264 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab133264 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

  • Immunoprecipitation - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Immunoprecipitation - Anti-ERG antibody [EPR3864(2)] (ab133264)

    ab133264 (purified) at 1/30 immunoprecipitating ERG in HEK293 cells (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Western blot - Anti-ERG antibody [EPR3864(2)] (ab133264)
    All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Western blot - Anti-ERG antibody [EPR3864(2)] (ab133264)
    All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/1000 dilution (unpurified)

    Lane 1 : Fli1 recombinant protein
    Lane 2 : ERG recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864(2)] (ab133264)

    Immunohistochemical analysis of paraffin embedded human prostate adenocarcinoma tissue labelling ERG with ab133264 at  a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-ERG antibody [EPR3864(2)] (ab133264)
    OI-RD Scanning - Anti-ERG antibody [EPR3864(2)] (ab133264)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ERG antibody [EPR3864(2)] (ab133264)
    Anti-ERG antibody [EPR3864(2)] (ab133264)

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