Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-MAGEA9 antibody [EPR16258] (ab191568)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16258] to MAGEA9
Suitable for: Flow Cyt, IP, WB
Reacts with: Human

Product name

Anti-MAGEA9 antibody [EPR16258]
See all MAGEA9 primary antibodies
Description

Rabbit monoclonal [EPR16258] to MAGEA9
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562 cell lysate: K562 cells; Human testis and melanoma lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16258
Isotype

IgG
Research areas

Western blot - Anti-MAGEA9 antibody [EPR16258] (ab191568)

Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution + K562 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Immunoprecipitation - Anti-MAGEA9 antibody [EPR16258] (ab191568)

Immunoprecipitation analysis of human testis tissue lysate labeling MAGEA9 using ab191568 at 1/100 dilution (Left Lane). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Right Lane: PBS instead of human testis tissue lysate.
Flow Cytometry - Anti-MAGEA9 antibody [EPR16258] (ab191568)

Flow cytometric analysis of 562 cells labeling MAGEA9 using ab191568 at 1/350 dilution (red) or rabbit monoclonal IgG as isotype control (green). Secondary antibody Goat anti rabbit IgG (FITC). Cells were fixed in 2% paraformaldehyde.
Western blot - Anti-MAGEA9 antibody [EPR16258] (ab191568)

All lanes : Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution

Lane 1 : Human testis lysate
Lane 2 : Human melanoma lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa

5% NFDM/TBST blocking buffer
Anti-MAGEA9 antibody [EPR16258] (ab191568)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com