Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAGEA9 antibody [EPR16258] (ab191568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16258] to MAGEA9
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MAGEA9 antibody [EPR16258]
    See all MAGEA9 primary antibodies

  • Description

    Rabbit monoclonal [EPR16258] to MAGEA9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562 cell lysate: K562 cells; Human testis and melanoma lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16258

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MAGEA9 antibody [EPR16258] (ab191568)
    Western blot - Anti-MAGEA9 antibody [EPR16258] (ab191568)
    Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution + K562 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

  • Immunoprecipitation - Anti-MAGEA9 antibody [EPR16258] (ab191568)
    Immunoprecipitation - Anti-MAGEA9 antibody [EPR16258] (ab191568)

    Immunoprecipitation analysis of human testis tissue lysate labeling MAGEA9 using ab191568 at 1/100 dilution (Left Lane). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Right Lane: PBS instead of human testis tissue lysate.

  • Flow Cytometry - Anti-MAGEA9 antibody [EPR16258] (ab191568)
    Flow Cytometry - Anti-MAGEA9 antibody [EPR16258] (ab191568)

    Flow cytometric analysis of 562 cells labeling MAGEA9 using ab191568 at 1/350 dilution (red) or rabbit  monoclonal IgG as isotype control (green). Secondary antibody Goat anti rabbit IgG (FITC). Cells were fixed in 2% paraformaldehyde.

  • Western blot - Anti-MAGEA9 antibody [EPR16258] (ab191568)
    Western blot - Anti-MAGEA9 antibody [EPR16258] (ab191568)
    All lanes : Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human melanoma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa



    5% NFDM/TBST blocking buffer

  • Anti-MAGEA9 antibody [EPR16258] (ab191568)
    Anti-MAGEA9 antibody [EPR16258] (ab191568)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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