Anti-MAGEC1 antibody [EPR18067] - BSA and Azide free (ab251470)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18067] to MAGEC1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MAGEC1 antibody [EPR18067] - BSA and Azide free
See all MAGEC1 primary antibodies -
Description
Rabbit monoclonal [EPR18067] to MAGEC1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251470 is the carrier-free version of ab207304. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251470 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR18067 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MAGEC1 antibody [EPR18067] (ab207304) at 1/5000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 124 kDa
Observed band size: 124,180 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis data was developed using ab207304, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-MAGEC1 antibody [EPR18067] (ab207304) at 1/5000 dilution + Human testis lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
Exposure time: 15 secondsThis data was developed using ab207304, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC1 antibody [EPR18067] - BSA and Azide free (ab251470)This data was developed using ab207304, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human testis tissue labeling MAGEC1 with ab207304 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human testis is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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