Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABCG1 antibody [EP1366Y] (ab52617)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1366Y] to ABCG1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Cow, Human

Overview

  • Product name

    Anti-ABCG1 antibody [EP1366Y]
    See all ABCG1 primary antibodies

  • Description

    Rabbit monoclonal [EP1366Y] to ABCG1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Cow
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HUVEC cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 20 µg

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat liver lysate
    Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
    Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

    Predicted band size: 75 kDa



    Blocking and diluting buffer: 5% NFDM /TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)

    ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

  • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antiibody at 1/2000 dilution

    Predicted band size: 75 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Anti-ABCG1 antibody [EP1366Y] (ab52617)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ABCG1 antibody [EP1366Y] (ab52617)

© 2021 Astana Biomed Group LLP. All rights reserved.