Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)
![Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)](https://www.abcam.com/ps/products/190/ab190368/Images/ab190368-225920-anti-aldolase-c-antibody-4a9-n-terminal-immunofluorescence.jpg "Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)")
Key features and details
- Mouse monoclonal [4A9] to Aldolase C - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Aldolase C antibody [4A9] - N-terminal
See all Aldolase C primary antibodies -
Description
Mouse monoclonal [4A9] to Aldolase C - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Pig
Predicted to work with: Rabbit, Horse, Chicken, Human, Chimpanzee, Cynomolgus monkey
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Immunogen
Synthetic peptide corresponding to Human Aldolase C aa 1-100 (N terminal).
Database link: P09972 -
Positive control
- WB: Rat and mouse brain lysate; Cow cerebellum lysate; Pig hippocampus lysate. ICC/IF: Rat cerebellum section and E20 rat cortical culture.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4A9 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)
Immunofluorescence analysis of neuron/glial cells, labeling Aldolase C using ab190368 at a 1/500 dilution (green). Counterstained with an antibody against NeuN (red) and DNA (blue).
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![Western blot - Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)](https://www.abcam.com/ps/products/190/ab190368/Images/ab190368-313162-anti-aldolase-c-antibody-4a9-n-terminal-western-blot.jpg)
Western blot - Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)All lanes : Anti-Aldolase C antibody [4A9] - N-terminal (ab190368) at 1/2000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : Cow cerebellum lysate
Lane 5 : Pig hippocampus lysate
Predicted band size: 39 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)](https://www.abcam.com/ps/products/190/ab190368/Images/ab190368-313161-anti-aldolase-c-antibody-4a9-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)Immunofluorescence analysis of rat cerebellum tissue (A) or E20 rat cortical culture (B) stained for Aldolase C using ab190368 at a 1/1000 dilution (green)). Costained with rabbit polyclonal to CFAP (red) at a 1/5000 dilution (A) or with chicken polyclonal antibody to MAP2 (red) at a 1/10000 dilution (B). DAPI was used to staing nuclear DNA (blue).
