Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Wednesday March 03, 2021

HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR9724(B)] to Aldolase + Aldolase B + Aldolase C
Suitable for: WB
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]
Description

HRP Rabbit monoclonal [EPR9724(B)] to Aldolase + Aldolase B + Aldolase C
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human Aldolase.
Positive control
- WB : A549 and HeLa whole cell lysates as well as Human fetal liver, fetal brain skeletal muscle tissue lysates. His-tagged human Aldolase A, B and C full-length recombinant protein.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9724(B)
Isotype

IgG
Research areas

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)

All lanes : Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab169544) at 1/1000 dilution

Lane 1 : His-tagged human Aldolase A full-length recombinant protein
Lane 2 : His-tagged human Aldolase B full-length recombinant protein
Lane 3 : His-tagged human Aldolase C full-length recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa
Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)

All lanes : HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774) at 1/5000 dilution

Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : Fetal Liver (Human) Normal Tissue)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Fetal Brain (Human) Normal Tissue lysate
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 4 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200774 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com