Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12743(B)] to ALDH1L1
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ALDH1L1 antibody [EPR12743(B)]
    See all ALDH1L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12743(B)] to ALDH1L1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ALDH1L1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75891

  • Positive control

    • HepG2, 293T, fetal brain, fetal kidney and fetal liver lysates. Human kidney and Human liver tissue. IHC-Fr: Mouse and rat liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Western blot - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    All lanes : Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : Fetal kidney lysate
    Lane 5 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 99 kDa

  • Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Immunohistochemistry (Frozen sections) analysis of mouse liver tissue labeling ALDH1L1 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Cytoplasmic staining on mouse liver.
  • Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Immunohistochemistry (Frozen sections) analysis of rat liver tissue labeling ALDH1L1 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Cytoplasmic staining on rat liver.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Western blot - Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    All lanes : Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463) at 1/1000 dilution

    Lane 1 : Fetal liver lysate at 10 µg
    Lane 2 : 1X PBS (negative control)

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 99 kDa

  • Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)
    Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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