Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16098] to TIMM17A/TIM17
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TIMM17A/TIM17 antibody [EPR16098]
    See all TIMM17A/TIM17 primary antibodies

  • Description

    Rabbit monoclonal [EPR16098] to TIMM17A/TIM17

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, K562, Raw264.7, PC-12 and NIH/3T3 cell lysates; HeLa cells.

  • General notes

     

     

     This product was previously labelled as TIMM17A

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)
    Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)
    All lanes : Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)
    Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)
    All lanes : Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)
    Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling TIMM17A/TIM17 with ab192246 at 1/50 dilution (11.7 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

    The two negative controls are ab192246 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

  • Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)
    Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)

    Western blot analysis of TIMM17A/TIM17 in HeLa cell lysates immunoprecipitated using ab192246 at 1/40 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate.

     

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)
    Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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