Anti-ALDH1L1 antibody (ab56777)
Key features and details
- Mouse monoclonal to ALDH1L1
- Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG3
Overview
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Product name
Anti-ALDH1L1 antibody
See all ALDH1L1 primary antibodies -
Description
Mouse monoclonal to ALDH1L1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP MouseWB Mouse
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Immunogen
Recombinant fragment: EESFGPVMII SRFADGDLDA VLSRANATEF GLASGVFTRD INKALYVSDK LQAGTVFVNT YNKTDVAAPF GGFKQSGFGK DLGEAALNEY LRVKTVTFEY , corresponding to amino acids 803-903 of Human ALDH1L1
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General notes
This product was changed from ascites to tissue culture supernatant on 22 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody (ab56777)
ALDH1L1 antibody (ab56777) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
This image was generated using the ascites version of the product.
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Western blot - Anti-ALDH1L1 antibody (ab56777)ALDH1L1 antibody (ab56777) at 1ug/lane + NIH/3T3 cell lysate at 25 μg/lane.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1 antibody (ab56777)ICC/IF image of ab56777 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56777, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-ALDH1L1 antibody (ab56777)Overlay histogram showing HepG2 cells stained with ab56777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56777, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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Immunoprecipitation - Anti-ALDH1L1 antibody (ab56777)ALDH1L1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to ALDH1L1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56777.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 99kDa: ALDH1L1; non specific - 60kDa: We are unsure as to the identity of this extra band.This image was generated using the ascites version of the product.
