Anti-Thrombospondin antibody (ab223580)
Key features and details
- Goat polyclonal to Thrombospondin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Thrombospondin antibody
See all Thrombospondin primary antibodies -
Description
Goat polyclonal to Thrombospondin -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human Thrombospondin aa 450-550 (internal sequence). (NP_003237.2).
Database link: P079962 -
Positive control
- WB: HEK-293 cells overexpressing human thrombospondin with C-terminal MYC tag.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223580 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Thrombospondin antibody (ab223580)Lanes 1-2 : Anti-Thrombospondin antibody (ab223580) at 1 µg/ml
Lane 3 : Anti-Myc tag at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate overexpressing human Thrombospondin with C-terminal Myc tag (in RIPA buffer)
Lane 2 : Mock-transfected HEK-293 cells lysate (in RIPA buffer)
Lane 3 : HEK-293 cell lysate overexpressing human Thrombospondin with C-terminal Myc tag (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 129 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Primary incubations were for 1 hour.
