Anti-P5CS antibody (ab225999)
Key features and details
- Rabbit polyclonal to P5CS
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P5CS antibody
See all P5CS primary antibodies -
Description
Rabbit polyclonal to P5CS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human P5CS aa 745-795. The exact sequence is proprietary.
Database link: P54886-2 -
Positive control
- IP: HEK-293T whole cell lysate. WB: HEK-293T, HeLa, HepG2 and MCF7 cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225999 was affinity purified using an epitope specific to P5CS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-P5CS antibody (ab225999)All lanes : Anti-P5CS antibody (ab225999) at 0.04 µg/ml
Lane 1 : HepG2 cell lysate prepared using NETN lysis buffer
Lane 2 : MCF-7 cell lysate prepared using NETN lysis buffer
Lane 3 : HeLa cell lysate prepared using NETN lysis buffer
Lane 4 : HEK293T cell lysate prepared using NETN lysis buffer
Lysates/proteins at 10 µg per lane.
Predicted band size: 87 kDaDetection: Chemiluminescence with an exposure time of 3 minutes.
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Immunoprecipitation - Anti-P5CS antibody (ab225999)P5CS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225999 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225999 at 0.4 µg/ml.
Lane 1: ab225999 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
