Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19382] to Argininosuccinate Lyase
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Argininosuccinate Lyase antibody [EPR19382]
    See all Argininosuccinate Lyase primary antibodies

  • Description

    Rabbit monoclonal [EPR19382] to Argininosuccinate Lyase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal heart, fetal kidney, fetal spleen and fetal liver lysates; HepG2 untreated and treated with 1 µM dexamethasone for 24 hours whole cell lysates; HEK-293 HeLa, T-47D, A549 and SH-SY5Y whole cell lysates. IP: Human fetal liver whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)
    Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)
    All lanes : Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal spleen lysate
    Lane 4 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2 and 3: 8 seconds; Lane 4: 1 second.

  • Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)
    Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)
    All lanes : Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025) at 1/1000 dilution

    Lane 1 : Untreated HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) treated with 1 µM dexamethasone for 24 hours whole cell lysate
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 5 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
    Lane 6 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 1 second; Lane 3,4 and 5: 15 seconds; Lane 6 and 7: 30 seconds.

    Dexamethasone treatment increase the accumulation of ASL mRNA (PMID: 2209616).

  • Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)
    Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)

    Argininosuccinate Lyase was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab201025 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab201025 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal liver whole cell lysate, 10µg (Input).

    Lane 2: ab201025 IP in Human fetal liver whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal-Isotype Control (ab172730) instead of ab201025 in Human fetal liver whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)
    Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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