Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 03, 2021

Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19396] to Argininosuccinate Lyase
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Argininosuccinate Lyase antibody [EPR19396]
See all Argininosuccinate Lyase primary antibodies
Description

Rabbit monoclonal [EPR19396] to Argininosuccinate Lyase
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver and testis lysates; HepG2, T-47D, A549, SH-SY5Y, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and Rat liver lysates. IP: Human fetal liver whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19396
Isotype

IgG
Research areas

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)

All lanes : Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/5000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human testis lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 5 : Mouse liver lysate
Lane 6 : Rat liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 51 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 2 seconds; Lane 2: 1 minute; Lane 3 and 4: 5 seconds; Lane 5 and 6: 1 second.
Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)

All lanes : Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/2000 dilution

Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)

All lanes : Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/2000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)

All lanes : Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/5000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 2 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Argininosuccinate Lyase is predominantly expressed in the liver, and weakly expressed in the lung (PMID: 8576237).
Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)

Argininosuccinate Lyase was immunoprecipitated from 0.35 mg of Human fetal liver whole cell lysate with ab201026 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab201026 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human fetal liver whole cell lysate, 10µg (Input).

Lane 2: ab201026 IP in Human fetal liver whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab201026 in Human fetal liver whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)

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