Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21752-214] to beta 2 Microglobulin - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free
    See all beta 2 Microglobulin primary antibodies

  • Description

    Rabbit monoclonal [EPR21752-214] to beta 2 Microglobulin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HepG2 cell lysates. Flow Cyt: HepG2 and HeLa cells. IP: HeLa cell lysate. IHC-P: Human spleen and kidney tissue.

  • General notes

    Ab237032 is the carrier-free version of ab218230. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab237032 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)
    Western blot - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)
    All lanes : Anti-beta 2 Microglobulin antibody [EPR21752-214] (ab218230) at 1/500 dilution

    Lane 1 : Wild-type HepG2 cell lysate
    Lane 2 : B2M knockout HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab218230).

    Lanes 1-4: Merged signal (red and green). Green - ab218230 observed at 14 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab218230 Anti-beta 2 Microglobulin antibody [EPR21752-214]  was shown to specifically react with beta 2 Microglobulin in wild-type HepG2 cells. Loss of signal was observed when knockout cell line ab262325 (knockout cell lysate ab256846) was used. Wild-type and beta 2 Microglobulin knockout samples were subjected to SDS-PAGE. ab218230 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling beta 2 Microglobulin with ab218230 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining on endothelial cells of human kidney is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218230).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)
    Flow Cytometry - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling beta 2 Microglobulin with ab218230 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG - Isotype control (ab172730) (black) and an unlabeled control cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218230).

  • Immunoprecipitation - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)
    Immunoprecipitation - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)

    Beta 2 Microglobulin was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab218230 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab218230 IP in HeLa whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218230 in HeLa whole cell lysate (-).


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218230).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling beta 2 Microglobulin with ab218230 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining on endothelial cells of human spleen is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218230).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Flow Cytometry - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)
    Flow Cytometry - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling beta 2 Microglobulin with ab218230 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG - Isotype control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218230).

  • Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)
    Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (ab237032)

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