Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)
![Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-269179-anti-beta-2-microglobulin-antibody-b2m-961-immunohistochemistry.jpg "Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)")
Key features and details
- Mouse monoclonal [B2M/961] to beta 2 Microglobulin
- Suitable for: WB, Flow Cyt, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-beta 2 Microglobulin antibody [B2M/961]
See all beta 2 Microglobulin primary antibodies -
Description
Mouse monoclonal [B2M/961] to beta 2 Microglobulin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human beta 2 Microglobulin aa 1 to the C-terminus.
Database link: P61769 -
Positive control
- IHC-P: Human cervical carcinoma, melanoma, endometrial carcinoma, renal carcinoma, bladder carcinoma and colon carcinoma tissues; WB: THP-1 and Raji cell lysates; ICC and Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab215889 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
B2M/961 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 µg/ml.
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![Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-456437-anti-beta-2-microglobulin-antibody-b2m-961-western-blot-thp1-raji-human.jpg)
Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)All lanes : Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889) at 2 µg/ml
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Predicted band size: 14 kDa
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![Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-456436-anti-beta-2-microglobulin-antibody-b2m-961-flow-cytometry-hela-human.jpg)
Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta 2 Microglobulin with ab215889 at 2 µg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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![Immunocytochemistry - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-456434-anti-beta-2-microglobulin-antibody-b2m-961-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta 2 Microglobulin with ab215889 at 4 µg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-269180-anti-beta-2-microglobulin-antibody-b2m-961-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling beta 2 Microglobulin with ab215889 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-269181-anti-beta-2-microglobulin-antibody-b2m-961-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrial carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-269182-anti-beta-2-microglobulin-antibody-b2m-961-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-269183-anti-beta-2-microglobulin-antibody-b2m-961-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-269184-anti-beta-2-microglobulin-antibody-b2m-961-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)](https://www.abcam.com/ps/products/215/ab215889/Images/ab215889-269185-anti-beta-2-microglobulin-antibody-b2m-961-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1µg/ml.
