Phosphatidylserine Assay Kit (Fluorometric) (ab273295)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 5 µM
Overview
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Product name
Phosphatidylserine Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
5 µM -
Assay duration
Multiple steps standard assay -
Product overview
Phosphatidylserine Assay Kit (Fluorometric) (ab273295) allows the quantification of Phosphatidylserine (PS) in lipid extracts of cell or tissue lysates and biological fluids (serum/plasma). The assay is based on the enzymatic cleavage of PS to yield phosphatidic acid and L-serine, which subsequently reacts with a probe to form a stable fluorophore (Ex/Em = 538/587 nm). The assay is selective for PS (other phospholipids such as phosphatidylcholine, phosphatidylethanolamine or phosphatidic acid do not interfere), is high-throughput adaptable and can detect as little as 50 pmole/well of PS (5 μM in a 10 μl sample volume).
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer Enzyme Mix (Lyophilized) 1 vial Lipase Enzyme Mix (Lyophilized) 1 vial Phosphatidylserine Assay Buffer 1 x 25ml Phosphatidylserine Standard (1 mM) 1 x 200µl Probe Solution 1 x 200µl Serine Enzyme Mix (Lyophilized) 1 vial -
Research areas
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Relevance
Phosphatidylserine, or PS, is a naturally occurring, phospholipid nutrient. PS is essential to the functioning of all the cells of the body, but is most concentrated in the brain. Its relative abundance in this organ reflects its proven involvement in an assortment of nerve cell functions, including nerve transmitter release and synaptic activity. Clinical studies have suggested that PS can support brain functions that tend to decline with age. -
Alternative names
- Phosphatidyl serine
- PS
Images
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Phosphatidylserine standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Specificity for detection of Phosphatidylserine (PS) over other common phospholipids.At a 5-fold molar excess (5 nmoles) versus PS (1 nmole), phosphatidic acid (PA) contributes ≤10% interference while Phosphatidylcholine (PC), Phosphatidylethanolamine (PE) and Sphingomyelin (SM) contribute ≤5%.
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Estimation of PS in lipid extracts of lysates from rat neocortex and 3T3-L1 cells.Estimation of PS in lipid extracts of lysates from rat neocortex (2 µL extract, equivalent to 200 µg wet brain tissue) and 3T3-L1 cells (2 µL extract, equivalent to 200000 cells). Lipid extraction in MTBE/MeOH/Saline was carried out as described above and the dried extract was re-suspended in 1% Triton X-100 (w/v) solution. Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.
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Estimation of PS in lipid extracts of lysates from pooled human serum.Estimation of PS in lipid extracts of lysates from pooled human serum (10 µl extract, equivalent to 20 µl serum). Lipid extraction in MTBE/MeOH/Saline was carried out as described above and the dried extract was re-suspended in 1% Triton X-100 (w/v) solution. Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.
