Antibodies, Proteins, Kits and Reagents for Life Science

Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7 - BSA and Azide free
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free
See all p60 CAF1/MPP7 primary antibodies
Description

Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Does not react with: Mouse
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, HeLa, 293T, and Caco2 cell lysates; IHC: Human colon and tonsil tissues. Flow Cyt: HeLa cells. IP: HeLa.
General notes
ab226065 is the carrier-free version of ab109442.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6105
Isotype

IgG
Research areas

Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)

All lanes : Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Caco-2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 61 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

This data was developed using ab109442, the same antibody clone in a different buffer formulation.
Flow Cytometry - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)

This data was developed using ab109442, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)

This data was developed using ab109442, the same antibody clone in a different buffer formulation.ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human colon tissue. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)

This data was developed using ab109442, the same antibody clone in a different buffer formulation.ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human tonsil tissue. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-p60 CAF1/MPP7 antibody [EPR6105] - BSA and Azide free (ab226065)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com