Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rab 6A antibody [EPR24472-24] - BSA and Azide free (ab280955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24472-24] to Rab 6A - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Rab 6A antibody [EPR24472-24] - BSA and Azide free
    See all Rab 6A primary antibodies

  • Description

    Rabbit monoclonal [EPR24472-24] to Rab 6A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICCmore details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC-3, HT-29, HCT116, 293T, A549, Neuro-2a, NIH/3T3, PC-12 and C6 whole cell lysares; Human brain, heart and kidney tissue lysates; Mouse brain, heart, kidney and spleen tissue lysates; Rat brain, heart, kidney and spleen tissue lysates. IHC-P: Human spleen tissue; Mouse testis tissue; Rat spleen tissue. ICC: A549 and Neuro-2a cells. Flow Cyt: A54 and Neuro-2a cells. IP: Neuro-2a and A549 whole cell lysates.

  • General notes

    ab280955 is the carrier-free version of ab271094. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280955 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24472-24

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Rab 6A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on mouse testis. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Immunocytochemistry - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling Rab 6A with ab271094 at 1/50 (11.84 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing golgi staining in Neuro-2a cell line. ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain GM130 at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Western blot - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    All lanes : Anti-Rab 6A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

    Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
    Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 6 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 7 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 9 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    This data was developed using ab271094, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 26 seconds.

     

  • Flow Cytometry - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Flow Cytometry - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling Rab 6A with ab271094 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Immunoprecipitation - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Rab 6A was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab271094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271094 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: ab271094 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271094 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling Rab 6A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on human spleen. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Immunocytochemistry - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A549 cells labelling Rab 6A with ab271094 at 1/50 (11.84 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing golgi staining in A549 cell line. ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain the GM-130 at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Western blot - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    All lanes : Anti-Rab 6A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

    Lanes 1-2 : Human brain tissue lysate
    Lane 3 : Human kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    This data was developed using ab271094, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1-2: 15 secondsLane 3: 48 seconds.

     

  • Flow Cytometry - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Flow Cytometry - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Rab 6A with ab271094 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Western blot - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    All lanes : Anti-Rab 6A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    This data was developed using ab271094, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 48 seconds.

     

  • Western blot - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Western blot - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    All lanes : Anti-Rab 6A antibody [EPR24472-24] (ab271094) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    This data was developed using ab271094, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1, 3, 4: 48 secondsLane 2: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling Rab 6A with ab271094 at 1/2000 (0.296 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining on rat spleen. The section was incubated with ab271094 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument .Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Immunoprecipitation - Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

    This data was developed using ab271094, the same antibody clone in a different buffer formulation.

    Rab 6A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab271094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271094 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab271094 IP in A549 whole cell lysate

    Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab271094 in A549 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

     

  • Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)
    Anti-Rab-6 antibody [EPR24472-24] - BSA and Azide free (ab280955)

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