Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [11445] to KIFC1 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-KIFC1 antibody [11445] - BSA and Azide free
    See all KIFC1 primary antibodies

  • Description

    Rabbit monoclonal [11445] to KIFC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2 and HAP1 cell lysates IHC-P: Human tonsil tissue, cervical carcinoma, stomachICC/IF: HeLa cells Flow Cyt: HeLa Cells IP: Jurkat whole cell lysates

  • General notes

    ab235994 is the carrier-free version of ab172620 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab235994 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Western blot - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: KIFC1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab172620 (unpurified) observed at 74 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab172620 was shown to specifically react with KIFC1 in wild-type cells as signal was lost in KIFC1 knockout cells. Wild-type and KIFC1 knockout samples were subjected to SDS-PAGE. Ab172620 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.228 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172620).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling KIFC1 with purified ab172620 at 1/16000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172620).

  • Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KIFC1 with purified ab172620 at 1/50 dilution (4.0 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172620).

  • Flow Cytometry - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Flow Cytometry - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KIFC1 with purified ab172620 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172620).

  • Immunoprecipitation - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Immunoprecipitation - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

    ab172620 (purified) at 1/20 dilution (1ug) immunoprecipitating KIFC1 in Jurkat whole cell lysates.
    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates 10ug
    Lane 2 (+): ab172620 & Jurkat whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172620 in Jurkat whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172620).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue laneling KIFC1 with ab172620 (unpurified) at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172620).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling KIFC1 with ab172620 (unpurified) at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172620).

  • Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

    Immunofluorescent staining of HeLa cells labeling KIFC1 with ab172620 (unpurified) at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172620).

  • Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)
    Anti-KIFC1 antibody [11445] - BSA and Azide free (ab235994)

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