Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Egr1 antibody [EPR15916] - BSA and Azide free (ab232448)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15916] to Egr1 - BSA and Azide free
Suitable for: WB, ICC/IF, IHC-P
Reacts with: Human

Product name

Anti-Egr1 antibody [EPR15916] - BSA and Azide free
See all Egr1 primary antibodies
Description

Rabbit monoclonal [EPR15916] to Egr1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human thyroid carcinoma tissue.
General notes
Ab232448 is the carrier-free version of ab194357. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232448 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15916
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Egr1 antibody [EPR15916] - BSA and Azide free (ab232448)

Confocal image showing nuclear and weakly cytoplasmic staining on Hela cells.

Immunocytometry/Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma ) cells labeling Egr1 with ab194357 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution. The cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei counterstained with DAPI (blue).

For negative control 1, ab194357 (1:200) was used, followed by (ab150120) anti-mouse secondary antibody at 1/1000 dilution. For negative control 2, (ab7291) mouse primary antibody (1/1000) was used followed by (ab150077) anti-rabbit secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194357).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody [EPR15916] - BSA and Azide free (ab232448)

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Egr1 with ab194357 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194357).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Egr1 antibody [EPR15916] - BSA and Azide free (ab232448)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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