Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Egr1 antibody [EPR5014(2)] (ab133695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5014(2)] to Egr1
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Egr1 antibody [EPR5014(2)]
    See all Egr1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5014(2)] to Egr1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Egr1 aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: P18146

  • Positive control

    • WB: HeLa, HeLa, THP-1, THP-1, NIH/3T3, and NIH/3T3 lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    Western blot - Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    All lanes : Anti-Egr1 antibody [EPR5014(2)] (ab133695) at 1/1000 dilution (Purified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 5% FBS for 1 hours after starving for 36 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Stimulation is required to allow detection of the target protein due to low levels of endogenous expression.

  • Western blot - Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    Western blot - Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    All lanes : Anti-Egr1 antibody [EPR5014(2)] (ab133695) at 1/5000 dilution (Purified)

    Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml LPS for 3 hours whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Stimulation is required to allow detection of the target protein due to low levels of endogenous expression in THP-1 cells which is consistent with what has been descirbed in PMID: 11520792

  • Western blot - Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    Western blot - Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    All lanes : Anti-Egr1 antibody [EPR5014(2)] (ab133695) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20ng/ml TNF-a for 60 minutes whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Stimulation is required to allow detection of the target protein due to low levels of endogenous expression in HeLa cells which is consistent with what has been descirbed in PMID: 20332214 

  • OI-RD Scanning - Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    OI-RD Scanning - Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Egr1 antibody [EPR5014(2)] (ab133695)
    Anti-Egr1 antibody [EPR5014(2)] (ab133695)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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