Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Liver Arginase antibody [BLR036F] (ab243892)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR036F] to Liver Arginase
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Human

Product name

Anti-Liver Arginase antibody [BLR036F]
See all Liver Arginase primary antibodies
Description

Rabbit monoclonal [BLR036F] to Liver Arginase
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Liver Arginase aa 280-322 (C terminal). The exact sequence is proprietary. NP_000036.2 and Gene ID 383.
Database link: P05089
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR036F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (ab243892)

Liver Arginase was immunoprecipitated from 1.0 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab243892 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243892 at 1/1000 dilution.
Lane 1: ab243892 IP in HepG2 whole cell lysate.

Lane 2: Control IgG in HepG2 whole cell lysate.

Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-Liver Arginase antibody [BLR036F] (ab243892)

Western blot analysis using ab243892 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: HEK-293T whole cell lysate (50 µg).
Lane 3: MCF7 whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: A549 whole cell lysate (50 µg).
Lane 6: SW 620 whole cell lysate (50 µg).
Lane 7: Jurkat whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry - Anti-Liver Arginase antibody [BLR036F] (ab243892)

Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Liver Arginase using ab243892 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (ab243892)

Formalin-fixed, paraffin-embedded human skin tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (ab243892)

Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-Liver Arginase antibody [BLR036F] (ab243892)

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