Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Liver Arginase antibody [EPR19652] (ab203490)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19652] to Liver Arginase
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Liver Arginase antibody [EPR19652]
    See all Liver Arginase primary antibodies

  • Description

    Rabbit monoclonal [EPR19652] to Liver Arginase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat liver lysate. IHC-P: Mouse and rat liver tissue. IHC-Fr: Mouse liver tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    All lanes : Anti-Liver Arginase antibody [EPR19652] (ab203490) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed in RAW 264.7 is consistent with the literatures (PMID: 28032600, PMID: 27166374, PMID: 9814991, PMID: 11080091).
    Negative control: RAW 264.7 (PMID: 28032600, PMID: 27166374, PMID: 9814991, PMID: 11080091).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat liver (PMID: 20661013; PMID: 21975054). Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Liver Arginase with ab203490 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in the hepatic cells of mouse liver (PMID: 21975054) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    All lanes : Anti-Liver Arginase antibody [EPR19652] (ab203490) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa


    Exposure time: 3 seconds


    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    Negative control: Mouse kidney (PMID: 21975054).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse liver (PMID: 20661013; PMID: 21975054). Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labeling Liver Arginase with ab203490 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Negative staining on mouse small intestine (PMID: 21975054) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control: No staining on mouse kidney (PMID: 21975054). Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Anti-Liver Arginase antibody [EPR19652] (ab203490) at 1/5000 dilution + Rat liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa


    Exposure time: 2 seconds


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Liver Arginase antibody [EPR19652] (ab203490)
    Anti-Liver Arginase antibody [EPR19652] (ab203490)

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