Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Liver Arginase antibody [EPR19652] (ab203490)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19652] to Liver Arginase
Suitable for: IHC-P, WB, IHC-Fr
Reacts with: Mouse, Rat

Product name

Anti-Liver Arginase antibody [EPR19652]
See all Liver Arginase primary antibodies
Description

Rabbit monoclonal [EPR19652] to Liver Arginase
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat liver lysate. IHC-P: Mouse and rat liver tissue. IHC-Fr: Mouse liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19652
Isotype

IgG
Research areas

Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)

All lanes : Anti-Liver Arginase antibody [EPR19652] (ab203490) at 1/1000 dilution

Lane 1 : Mouse liver tissue lysate
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

The expression profile observed in RAW 264.7 is consistent with the literatures (PMID: 28032600, PMID: 27166374, PMID: 9814991, PMID: 11080091).
Negative control: RAW 264.7 (PMID: 28032600, PMID: 27166374, PMID: 9814991, PMID: 11080091).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat liver (PMID: 20661013; PMID: 21975054). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Liver Arginase with ab203490 at 1/100 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in the hepatic cells of mouse liver (PMID: 21975054) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)

All lanes : Anti-Liver Arginase antibody [EPR19652] (ab203490) at 1/1000 dilution

Lane 1 : Mouse liver lysate
Lane 2 : Mouse kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa

Exposure time: 3 seconds

Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

Negative control: Mouse kidney (PMID: 21975054).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse liver (PMID: 20661013; PMID: 21975054). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labeling Liver Arginase with ab203490 at 1/100 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Negative staining on mouse small intestine (PMID: 21975054) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] (ab203490)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control: No staining on mouse kidney (PMID: 21975054). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Liver Arginase antibody [EPR19652] (ab203490)

Anti-Liver Arginase antibody [EPR19652] (ab203490) at 1/5000 dilution + Rat liver lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa

Exposure time: 2 seconds

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Anti-Liver Arginase antibody [EPR19652] (ab203490)

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