Anti-Liver Arginase antibody (ab96183)
Key features and details
- Rabbit polyclonal to Liver Arginase
- Suitable for: WB, IHC-P, ICC/IF, ELISA, IHC-Fr, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Liver Arginase antibody
See all Liver Arginase primary antibodies -
Description
Rabbit polyclonal to Liver Arginase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA HumanFlow Cyt HumanICC/IF HumanIHC-Fr MouseIHC-P MouseHumanIP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment corresponding to Human Liver Arginase aa 1-292.
Database link: P05089 -
Positive control
- WB: Raji whole cell lysate. Rat liver tissue extract. Mouse liver extract. IHC-P: Cal27 xenograft. Mouse liver tissue. ICC/IF: HeLa cells. IHC-Fr: Mouse hippocampus tissue. ELISA: HEK-293T whole cell extracts. Flow Cyt: HepG2 cell. IP: HepG2 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody (ab96183)
Frozen sections of mouse hippocampus tissue stained for Arginase 1 (green) using ab96183 at 1/250 dilution in immunohistochemical analysis. Counterstained with Fluoroshield with DAPI (blue).
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Western blot - Anti-Liver Arginase antibody (ab96183)Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution + Mouse liver extract at 50 µg
Predicted band size: 35 kDaSeparated by 12% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (ab96183)Paraffin embedded mouse liver tissue stained for Arginase 1 using ab96183 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Liver Arginase antibody (ab96183)Arginase 1 was immunoprecipitated from 5µ of HepG2 whole cell lysate with ab96183. Western blot was performed from the immunoprecipitate using ab96183 at 1/600 dilution. EasyBlot anti-Rabbit IgG, was used as secondary antibody.
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Flow Cytometry - Anti-Liver Arginase antibody (ab96183)Flow cytometric analysis of HepG2 cell line labeling ARG1 with ab96183 at 1/50 dilution (red) compared with Isotype Control (black) and an unlabelled control. Dylight 488-conjugated was used as secondary antibody. -
ELISA - Anti-Liver Arginase antibody (ab96183)ELISA analysis of HEK-293T whole cell extracts using ab96183 as a detection antibody with concentration of 1 μg/mL.
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Western blot - Anti-Liver Arginase antibody (ab96183)Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution + Rat liver tissue extract at 50 µg
Predicted band size: 35 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver Arginase antibody (ab96183)ab96183, at 1/500 dilution, staining liver Arginase in paraffin-embedded Cal27 xenograft by Immunohistochemistry. -
Western blot - Anti-liver Arginase antibody (ab96183)Anti-Liver Arginase antibody (ab96183) at 1/3000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-liver Arginase antibody (ab96183)ab96183, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
